Gluconate dehydratase enzymes and recombinant cells
AssigneeBP Corporation North America Inc.
InventorFernando Valle
Life of this patent
Estimating the term…
Abstract
Abstract: Gluconate dehydratase enzymes and recombinant cells are provided, along with their use in the production of 2-ke-to-3-deoxy-D-gluconate (KDG).
Claims25
A recombinant cell comprising a gene encoding a heterologous gluconate dehydratase, wherein the recombinant cell further comprises one or more genetic modifications resulting in at least one, any two, any three, any four, or all five of the following phenotypes:
- (a) decrease or elimination of glucose assimilation;
- (b) decrease or elimination of gluconate-6-phosphate production from gluconate;
- (c) decrease or elimination of glucose-6-phosphate production from glucose;
- (d) decrease or elimination of 2-keto-3-deoxy-D-gluconate (KDG) phosphorylation;
- and
- (e) decrease or elimination of KDG degradation by non-phosphorylative cellular reactions that consume KDG.
A recombinant cell comprising a genetic modification resulting in the decrease or elimination of 2-keto-3-deoxy-D-gluconate phosphorylation, wherein the recombinant cell further comprises one or more genetic modifications resulting in at least one, any two, any three, or all four of the following phenotypes:
- (a) decrease or elimination of glucose assimilation;
- (b) decrease or elimination of gluconate-6-phosphate production from gluconate;
- and
- (c) decrease or elimination of glucose-6-phosphate production from glucose;
- and
- (d) decrease or elimination of KDG degradation by non-phosphorylative cellular reactions that consume KDG.
3-53. (canceled)
A method of producing 2-keto-3-deoxy gluconate (KDG) comprising the steps of:
- (a) culturing a recombinant cell in a suitable culture medium, wherein the recombinant cell comprises a gene encoding a heterologous gluconate dehydratase enzyme having at least 70% sequence identity to SEQ ID NO:
- 1;
- and
- (b) allowing expression of said gene, wherein said expression results in the production of KDG;
- wherein said production of KDG is performed at a temperature between 20° C. and 45° C.
59-60. (canceled)
63-64. (canceled)
71-93. (canceled)
A nucleic acid comprising a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:
- 2. or SEQ ID NO:
- 3 or SEQ ID NO:
- 4, wherein the nucleic acid is isolated, recombinant or synthetic.
(canceled)
97-103. (canceled)
Dependencies are inferred from the claim text.