Recombinant gas vesicle nanoparticles
AssigneePukyong National University Industry-University Cooperation Foundation
InventorsJong-Myoung KIM, Shiladitya DASSARMA, Priya DASSARMA
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Abstract
The present disclosure relates to a recombinant gas vesicle nanoparticle, and the recombinant gas vesicle nanoparticle prepared by the method of the present disclosure is a nano-sized protein particle that it is biologically non-toxic and safe. Further, its outer surface protein, GvpC, can be recombined to include the exogenous protein to reconstitute gas vesicle nanoparticles presenting/mounting the exogenous protein on the surface, so that it can be used as various antigen-loaded vaccine compositions that are active under physiological conditions, therapeutic target-specific carriers, including a sensor for target-specific therapy.
Claims13
A method for preparing a recombinant gas vesicle nanoparticle (GVNP), the method comprising steps of:
- a) culturing Halobacterium sp.;
- b) collecting a gas vesicle (GV);
- c) removing GvpC from the gas vesicle;
- d) preparing a recombinant protein as an exogenous recombinant protein in which a full length or fragment of GvpC and an exogenous protein are fused;
- and
- e) reacting the gas vesicle from which GvpC has been removed with the exogenous recombinant protein to form a recombinant GVNP complex.
Dependencies are inferred from the claim text.