Rapid generation of plants with desired traits

A method of making an avocado plant cell comprising a loss of function mutation in polyphenol oxidase A (PPO-A), said method comprising:

• isolating nucellar tissue from an avocado plant;
• deriving a protoplast cell from the nucellar tissue;
• transfecting the protoplast cell with genome editing components;
• editing the protoplast cell genome to induce loss of function mutations in polyphenol oxidase A (PPO-A);
• and
• culturing the protoplast cell to make an avocado plant cell comprising a loss of function mutation in polyphenol oxidase A (PPO-A).

A method of making an avocado plant cell comprising altered expression of a gene of choice, said method comprising:

• isolating nucellar tissue from an avocado plant;
• deriving a protoplast cell from the isolated nucellar tissue;
• transfecting the protoplast cell with gene editing components to edit the protoplast cell genome to alter the expression of a gene;
• and
• culturing the protoplast cell to make an avocado plant cell comprising altered expression of the gene.