Polypeptide having cephalosporin c acylase activity and use thereof

A mutant cephalosporin C (CPC) acylase, derived from a CPC acylase comprising an alpha-subunit and a beta-subunit,

• wherein the mutant CPC acylase comprises a mutation by at least one amino acid substitution selected from the group consisting of:
• substitution of alanine at position 11 in the alpha-subunit of SEQ ID NO:
• 3 (A11α) with asparagine (N), proline (P), glutamine (Q), isoleucine (I), valine (V), or leucine (L);
• substitution of glycine at position 24 in the alpha-subunit of SEQ ID NO:
• 3 (G24α) with an amino acid different from the original amino acid residue;
• substitution of alanine at position 136 in the beta-subunit of SEQ ID NO:
• 4 (A136β) with an amino acid different from the original amino acid residue;
• substitution of isoleucine at position 179 in the beta-subunit of SEQ ID NO:
• 4 (I179β) with an amino acid different from the original amino acid residue;
• and
• substitution of histidine at position 453 in the beta-subunit of SEQ ID NO:
• 4 (H453β) with an amino acid different from the original amino acid residue.