Mutant transglutaminase

A mutant transglutaminase, having a specific mutation in the amino acid sequence of a wild-type transglutaminase, and having a transglutaminase activity,

• wherein the specific mutation is a mutation of improving thermostability and/or pH stability,
• wherein the specific mutation comprises the mutation (A) and/or the mutation (B) shown below:
• the mutation (A):
• mutation(s) at one or more amino acid residues selected from the followings:
• G275, D1, Y24, R48, S101, G102, N139, D142, L147, K152, G157, R167, N176, K181, E182, H188, D189, R208, T245, S246, G250, S284, H289, G301, and K327;
• the mutation (B):
• a mutation of introducing a disulfide bond, and
• wherein the mutant transglutaminase satisfies (B1) and/or (A1) shown below:
• (A1) the specific mutation comprises the mutation (A), and the mutation (A) comprises mutation(s) at one or more amino acid residues selected from the followings:
• D1, R48, G102, N139, D142, L147, K152, R167, N176, K181, E182, D189, S246, H289, G301, and K327,
• provided that the mutation at R48 is R48I when the mutation at R48 is solely selected, and
• provided that the mutation at S246 is S246N when the mutation at S246 is solely selected;
• (B1) the specific mutation comprises the mutation (B), and the mutation (B) comprises a combination of D3C/G283C and one or more mutations selected from E93CN112C, A81C/V311C, A106C/D213C, E107C/Y217C, A160C/G228C, S84C/K121C, R79C/P169C, A113C/P220C, E119C/S299C, and R89C/S116C.