Mushroom line n-s34, incorporated into hybrid mushroom strain la3782, and derivatives thereof

An Agaricus bisporus culture comprising a complete set of chromosomes of Agaricus bisporus line N-s34, a representative culture of said line N-s34 having been deposited under CNCM Accession Number I-5528, wherein said set of chromosomes comprises the sequence-characterized allelic markers listed in Table I.

An Agaricus bisporus culture comprising a haploid set of chromosomes of strain LA3782, a representative culture of said strain LA3782 having been deposited under CNCM Accession Number I-5527, said haploid set of chromosomes comprising the sequence-characterized allelic markers listed in Table II, provided that said culture is not strain BP-1, a representative culture of said strain BP-1 having been deposited under ATCC Accession Number PTA-6903.

An Agaricus bisporus culture that is derived from an initial culture, wherein said initial culture is selected from the group consisting of:

• a) strain LA3782, a representative culture of said strain LA3782 having been deposited under CNCM Accession Number I-5527,
• b) line N-s34, a representative culture of said line N-s34 having been deposited under CNCM Accession Number I-5528.

The method of claim 36, wherein said new Agaricus bisporus culture is characterized in that:

• (a) yield performance of crops produced from said new Agaricus bisporus culture is equal to or exceeds yield performance of crops of a BR06/Heirloom strain of Agaricus bisporus, and
• (b) a third-flush yield of crops produced from said new Agaricus bisporus culture exceeds that of the BR06/Heirloom strain, and
• (c) mushroom product of crops produced from said new Agaricus bisporus culture retains more weight after a number of days of post-harvest storage at 4 degrees Celsius than does mushroom product of the BR06/Heirloom strain, the number of days selected from the group comprising 3, 4, 5, 6, 7, and 8 days.