# US 2023/0263113 A1 · Seedling, method for growing seedling, compost, and method for growing plant > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0263113 A1 - Document ID: US20230263113A1 - Status: Published application - Kind: A1 - Application number: 18/002,375 - Filed: 2021-10-25 - Published: 2023-08-24 - Earliest claimed: 2020-11-12 - Assignees: Sunlit Seedlings Ltd. - Applicants: Sunlit Seedlings Ltd. - Inventors: Sota ISHIKAWA - Web page: https://patentgrep.com/patent/US20230263113A1 ## CPC - A01G 23/00: Forestry - A01G 2/00: Vegetative propagation (plant reproduction by tissue culture techniques A01H4/00) - A01G 7/00: Botany in general - A01G 18/10: Mycorrhiza; Mycorrhizal associations - A01G 18/20: Culture media, e.g. compost - A01G 18/50: Inoculation of spawn - A01G 24/22: containing plant material - A01H 17/00: Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) - A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom - A01P 21/00: Plant growth regulators - Y02P 60/40: Afforestation or reforestation - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract A method for growing a seedling, the method includes specifying a growth-contributing fungus contributable to growth of a target plant to be grown artificially, isolating the growth-contributing fungus, culturing the isolated growth-contributing fungus to obtain an inoculum, growing the inoculum with a growth substrate to obtain a microorganism bed, and rooting and growing the target plant in compost including the microorganism bed. In the specifying a growth-contributing fungus contributable to growth of a target plant to be grown artificially, the growth-contributing fungus is an indigenous nonmycorrhizal filamentous fungus to inhabit a planting site in which the target plant is to be planted or a neighborhood of the planting site and to live in symbiosis with a plant without a mycorrhiza. ## Claims ### Claim 1 A method for growing a seedling, the method comprising: specifying a growth-contributing fungus contributable to growth of a target plant to be grown artificially, the growth-contributing fungus being an indigenous nonmycorrhizal filamentous fungus to inhabit a planting site in which the target plant is to be planted or a neighborhood of the planting site and to live in symbiosis with a plant without a mycorrhiza; isolating the growth-contributing fungus; culturing the isolated growth-contributing fungus to obtain an inoculum; growing the inoculum with a growth substrate to obtain a microorganism bed; and rooting and growing the target plant in compost including the microorganism bed. ### Claim 2 (depends on 1) The method according to claim 1, wherein the seedling is a containerized tree seedling obtained by rooting and growing the target plant in a container containing the compost. ### Claim 3 (depends on 1) The method according to claim 1, wherein the seedling is a coniferous seedling for afforestation. ### Claim 4 (depends on 1) The method according to claim 1, wherein the growth-contributing fungus is a nonmycorrhizal filamentous fungus isolated from indigenous nonmycorrhizal filamentous fungi inhabiting the planting site or the neighborhood. ### Claim 5 (depends on 1) A seedling obtained by with the method according to claim 1. ### Claim 6 (depends on 4) A seedling obtained with the method according to claim 4. ### Claim 7 (depends on 3) A seedling obtained with the method according to claim 3. ### Claim 8 (depends on 5) A method for growing a plant, the method comprising: planting and growing the seedling according to claim 5 in the planting site. ### Claim 9 Compost for growing a plant artificially, the compost comprising: a microorganism bed in which an inoculum obtained by isolating and culturing a growth-contributing fungus is grown with a growth substrate for the inoculum, the growth-contributing fungus being a nonmycorrhizal filamentous fungus to live in symbiosis with a plant without a mycorrhiza and being contributable to growth of a target plant to be grown artificially; and organic matter usable by the growth-contributing fungus included in the microorganism bed to extend a mycelium. ### Claim 10 (depends on 9) The compost according to claim 9, wherein the growth-contributing fungus is an indigenous nonmycorrhizal filamentous fungus inhabiting a planting site in which the target plant is to be planted or a neighborhood of the planting site. ### Claim 11 (depends on 9) The compost according to claim 9, wherein the compost is usable to root and grow the target plant in the compost to be a seedling. ### Claim 12 A method for growing a plant, the method comprising: specifying a symbiotic microorganism to promote growth of a target plant; and placing compost into rhizosphere in which a root of the target plant extends, the compost including a microorganism bed in which the symbiotic microorganism is grown. ### Claim 13 (depends on 12) The method according to claim 12, wherein the symbiotic microorganism is a filamentous fungus to extend a mycelium of the symbiotic microorganism in the compost, and the compost is placed in the rhizosphere with the mycelium of the symbiotic microorganism extending in the compost to allow the root of the target plant to extend. ### Claim 14 (depends on 9) A method for growing a plant, the method comprising: placing the compost according to claim 9 into rhizosphere in which a root of the target plant extends.