Kit for detecting dust mite component-specific antibodies

A kit for detecting dust mite component-specific antibodies, comprising:

• a biotin-polystreptavidin-biotin-dust mite antigen-coated nitrocellulose (NC) membrane, a washing solution, an alkaline phosphatase (ALP)-labeled secondary antibody solution for dust mite component-specific antibodies, and a substrate solution;
• wherein the biotin-polystreptavidin-biotin-dust mite antigen-coated NC membrane is formed by coating an NC membrane sequentially with solidified biotin, polystreptavidin, and biotin-labeled dust mite antigens;
• the dust mite antigens comprise:
• dust mite protein Der f 1, dust mite protein Der f 2, dust mite protein Der p 1, dust mite protein Der p 2, dust mite protein Der p 5, dust mite protein Der p 7, dust mite protein Der p 10, dust mite protein Der p 11, dust mite protein Der p 20, dust mite protein Der p 21, dust mite protein Der p 23, dust mite protein Blo t 5, dust mite protein Blo t 10, dust mite protein Blo t 21, Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Blomia tropicalis;
• and the dust mite antigens are coated at different positions on the NC membrane;
• the dust mite component-specific antibodies comprise sIgE and sIgG, and sIgG comprises sIgG4;
• a preparation method of the biotin-polystreptavidin-biotin-dust mite antigen-coated NC membrane comprises the following steps:
• coating biotin on an NC membrane, and drying;
• coupling polystreptavidin to biotin, and drying;
• and coupling biotin-labeled dust mite antigens to polystreptavidin;
• and
• a preparation method of the ALP-labeled secondary antibody solution for dust mite component-specific antibodies comprises:
• mixing ALP with a secondary antibody for dust mite component-specific antibodies to obtain the ALP-labeled secondary antibody solution for dust mite component-specific antibodies.