Extracellular vesicle purification material and purification method

Abstract

The present disclosure relates to an extracellular vesicle purification material and purification method. The purification method adopts metal oxide microspheres or magnetic beads that can reversibly bind to phosphatidylserine to purify extracellular vesicles, and the purification material comprises nano zirconium dioxide microspheres, nano titanium dioxide microspheres or nano aluminum oxide microspheres, nano zirconium dioxide magnetic microspheres, nano titanium dioxide magnetic microspheres or nano aluminum oxide magnetic microspheres, and porous zirconium dioxide nano microspheres, porous titanium dioxide nano microspheres or porous aluminum oxide nano microspheres. The purification method of the present disclosure can quickly purify multiple samples: serum/plasma, urine and cell culture supernatant, has a moderate flux so as to retain extracellular vesicles to the greatest extent, and can obtain high-purity extracellular vesicles, without chelating agents and other reagents that may affect subsequent experiments, via elution.

Dependencies are inferred from the claim text.