Digital molecular assays
AssigneeIlytica LLC
InventorJay T. GROVES
Life of this patent
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Abstract
Provided herein are systems, devices and methods for the rapid and accurate measurement of analytes by assay of binding events, by direct, digital measurement of individually resolved analyte/reporter binding events. The digital molecular assay systems, devices and methods disclosed herein are capable of particle-by-particle readout using optical reporter molecules that detect and report the binding of a single analyte molecule, and report each such binding in binary format. Such digital molecular assay systems, devices and methods are useful in a variety of applications, such as on mobile electronic devices for use in the field.
Claims22
1-43. (canceled)
A digital assay system for determining a concentration of analyte in a sample, comprising:
- a microprocessor;
- memory;
- image analysis software stored in the memory and comprising instructions being executable by the microprocessor to perform a method, the method comprising:
- analyzing an image of a plurality of signals emitted by at least one type of optical reporter molecules incubated with at least one type of analyte molecules, and
- digitally classifying the plurality of signals as active or null;
- and
- optionally, a communication interface.
45-65. (canceled)
Dependencies are inferred from the claim text.