Recombinant proteins for quantification of protein levels

A recombinant protein comprising a set of polypeptides having the amino acid sequences of SEQ ID NO:

• 2, SEQ ID NO:
• 3, SEQ ID NO:
• 4, SEQ ID NO:
• 5, SEQ ID NO:
• 6, SEQ ID NO:
• 7, SEQ ID NO:
• 8, SEQ ID NO:
• 9, SEQ ID NO:
• 10, SEQ ID NO:
• 11, SEQ ID NO:
• 12, SEQ ID NO:
• 13, SEQ ID NO:
• 14, SEQ ID NO:
• 15, SEQ ID NO:
• 16, SEQ ID NO:
• 17, SEQ ID NO:
• 18, SEQ ID NO:
• 19, SEQ ID NO:
• 20, SEQ ID NO:
• 21, SEQ ID NO:
• 22, SEQ ID NO:
• 23, SEQ ID NO:
• 24, SEQ ID NO:
• 25, SEQ ID NO:
• 26, SEQ ID NO:
• 27, SEQ ID NO:
• 28, SEQ ID NO:
• 29, SEQ ID NO:
• 30, SEQ ID NO:
• 31, SEQ ID NO:
• 32, SEQ ID NO:
• 33, SEQ ID NO:
• 34, SEQ ID NO:
• 35, SEQ ID NO:
• 36, SEQ ID NO:
• 37, SEQ ID NO:
• 38, SEQ ID NO:
• 39, SEQ ID NO:
• 40, SEQ ID NO:
• 41, SEQ ID NO:
• 42, and SEQ ID NO:
• 43.

A recombinant protein comprising a set of non-identical, contiguous polypeptides, the set comprising:

• a polypeptide consisting of a sequence present in P85A;
• a polypeptide consisting of a sequence present in P85B;
• a polypeptide consisting of a sequence present in PK3CA;
• a polypeptide consisting of a sequence present in PK3CAE545K;
• a polypeptide consisting of a sequence present in PK3CAH1047K;
• a polypeptide consisting of a sequence present in PK3CD;
• a polypeptide consisting of a sequence present in PK3CB;
• a polypeptide consisting of a sequence present in ERBB2;
• a polypeptide consisting of a sequence present in EGFR;
• a polypeptide consisting of a sequence present in RRAS2;
• and
• a polypeptide consisting of a sequence present in P55G,
• wherein the recombinant protein comprises a trypsin cleavage site between each polypeptide of the set that allows separation of each polypeptide upon exposure of the recombinant protein to trypsin.

A recombinant protein comprising a set of polypeptides having the amino acid sequences of SEQ ID NO:

• 45, SEQ ID NO:
• 46, SEQ ID NO:
• 47, SEQ ID NO:
• 48, SEQ ID NO:
• 49, SEQ ID NO:
• 50, SEQ ID NO:
• 51, SEQ ID NO:
• 52, SEQ ID NO:
• 53, SEQ ID NO:
• 54, SEQ ID NO:
• 55, SEQ ID NO:
• 56, SEQ ID NO:
• 57, SEQ ID NO:
• 58, SEQ ID NO:
• 59, SEQ ID NO:
• 60, SEQ ID NO:
• 61, SEQ ID NO:
• 62, SEQ ID NO:
• 63, SEQ ID NO:
• 64, SEQ ID NO:
• 65, SEQ ID NO:
• 66, SEQ ID NO:
• 67, SEQ ID NO:
• 68, SEQ ID NO:
• 69, SEQ ID NO:
• 70, SEQ ID NO:
• 71, SEQ ID NO:
• 72, SEQ ID NO:
• 73, SEQ ID NO:
• 74, SEQ ID NO:
• 75, SEQ ID NO:
• 82, SEQ ID NO:
• 83, SEQ ID NO:
• 84, SEQ ID NO:
• 85, SEQ ID NO:
• 86, and SEQ ID NO:
• 87.