Control of meiotic crossover in maize
AssigneesCold Spring Harbor Laboratory, Cambridge Enterprise Limited
InventorsRobert Martienssen, Charles Underwood, Ian Henderson
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods for increasing meiotic recombination in crop plants, as well as plants and seeds produced by such methods.
Claims26
An F1 hybrid maize seed, comprising
- an expression cassette comprising a maize promoter that is active during meiosis and operably linked to a nucleic acid encoding an artificial microRNA (amiRNA) that targets ZMET5 (SEQ ID NO:
- 37).
An F1 hybrid maize seed produced by crossing two different maize inbred lines, one of which comprises an expression cassette that includes a maize promoter that is active during meiosis and is operably linked to a nucleic acid encoding an artificial microRNA (amiRNA) that targets ZMET5 (SEQ ID NO:
- 37).
(canceled)
6-8. (canceled)
10-11. (canceled)
An F1 hybrid maize plant, comprising
- an expression cassette comprising a maize promoter that is active during meiosis and operably linked to a nucleic acid encoding an artificial microRNA (amiRNA) that targets ZMET5 (SEQ ID NO:
- 37).
(canceled)
A method, comprising:
- (a) introducing into an embryo of a maize plant or a cell of a maize plant an expression cassette that includes a maize promoter that is active during meiosis and is operably linked to a nucleic acid encoding an artificial microRNA (amiRNA) that targets ZMET5 (SEQ ID NO:
- 37) to produce an embryo comprising the expression cassette or a cell comprising the expression cassette;
- (b) regenerating the embryo comprising the expression cassette or the cell comprising the expression cassette of (a) to produce a T0 maize plant;
- and
- (c) crossing the T0 maize plant of (b) to a maize plant to produce F1 maize seed.
Dependencies are inferred from the claim text.