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US 2023/0235316 A1Published application
Model for insulin resistance
AssigneeChildren's Hospital Medical Center
InventorsTakanori Takebe, Masaki Kimura
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AssigneeChildren's Hospital Medical Center
InventorsTakanori Takebe, Masaki Kimura
Estimating the term…
Disclosed herein are insulin resistance reporters for use in quantifying insulin response in biological cells. These biological cells may be stem cell compositions or derivatives thereof comprising the insulin resistance reporter. The stem cell derivatives include but are not limited to insulin responsive cells, tissues, or organoids, such as pancreatic, brain, adipose, muscle, or liver cells, or tissues or organoids thereof. Also disclosed herein are methods of using said insulin resistance reporters and cells with these insulin resistance reporters as models to examine insulin resistance and screening for compounds that are potentially useful for the treatment of diseases or disorders associated with insulin resistance. The cells comprising an insulin resistance reporter may be hepatic cells or liver organoid compositions, which can be used in investigating hepatic insulin resistance, for example, as a result of non-alcoholic fatty liver disease or steatohepatitis.
A liver organoid comprising an insulin resistance reporter, wherein the insulin resistance reporter is operatively linked to an insulin-dependent gene of the liver organoid.
A insulin responsive cell, tissue, or organoid comprising an insulin resistance reporter, wherein the insulin resistance reporter is operatively linked to an insulin-dependent gene of the insulin responsive cell, tissue, or organoid.
An insulin resistance reporter, comprising one or more reporter genes flanked by a 5′ homology region and a 3′ homology region associated with an insulin-dependent gene.
An in vitro method of screening for candidate compounds for the treatment of a disease or disorder associated with insulin dysfunction, comprising:
A method of monitoring insulin response in a subject, comprising:
Dependencies are inferred from the claim text.