Genetically Modified Bovine Herpesvirus Type 1 (BHV-1) for use to Treat Cancer
OwnerKaren Mossman, Maria Davola +2inventors
InventorsKaren Mossman, Maria Davola, Susan Collins, Breanne Cuddington
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Abstract
A recombinant BHV-1 oncolytic virus is provided comprising a BHV-1 mutant with enhanced cancer selectivity and/or enhance immunostimulatory activity as compared to wildtype BHV-1. The BHV-1 mutant is genetically modified to express one or more immunomodulatory molecules that induce an anti-tumor immune response. A method of generating the recombinant BHV-1 oncolytic virus is also provided.
Claims30
A recombinant BHV-1 oncolytic virus comprising a BHV-1 mutant genetically modified to express a gene encoding an immunomodulatory molecule that induces an anti-tumor immune response, wherein one or more target genes in the BHV-1 mutant is at least partially deleted or altered to yield a mutant that exhibits enhanced cancer selectivity and/or enhanced immunostimulatory activity in comparison to wild type BHV-1.
A method of generating a recombinant BHV-1 oncolytic virus comprising the steps of:
- i) nucleofecting a cell-line with a vector expressibly incorporating a gene that encodes an immunomodulatory molecule that induces an anti-tumor immune response, wherein said gene is incorporated within a BHV-1 target gene;
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- ii) infecting the cell-line with wild type BHV-1 under suitable conditions to yield a recombinant BHV-1 oncolytic virus in which the target gene is at least partially deleted or altered and the immunomodulatory molecule is expressed, and the BHV-1 oncolytic virus exhibits enhanced cancer selectivity and/or enhanced immunostimulatory activity in comparison to wild type BHV-1.
A method of treating an individual with cancer comprising administering to the individual a BHV-1 mutant in which one or more BHV-1 target genes are partially or totally deleted or altered to yield a mutant that exhibits enhanced cancer selectivity and/or immunostimulatory activity in comparison to wild type BHV-1.
Dependencies are inferred from the claim text.