Methods for the improved formation of acarbose
AssigneeBayer Aktiengesellschaft
InventorsAlfred Puhler, Jorn KALINOWSKI, Susanne SCHNEIKER-BEKEL, Marcus PERSICKE, Lena SCHAFFERT
Life of this patent
Estimating the term…
Abstract
The present invention relates to Actinomycetales strains for the improved formation of acarbose. Provided are Actinomycetales strains which are engineered to overexpress dTDP-D-glucose-4,6-dehydratase (AcbB) and/or uridyltransferase (GtaB). Also provided are Actinomycetales strains which are engineered to have a reduced or absent expression of the small carbohydrate binding protein (Cgt) and/or a reduced or absent expression of genes which are essential for carotenoid synthesis. Also provided are tools, methods and means to generate these strains.
Claims15
A method to engineer an Actinomycetales strain, preferably an Actinoplanes strain, for the improved production of acarbose, the method comprising engineering the Actinomycetales strain
- (i) for absent or reduced expression of extracellular small carbohydrate binding protein Cgt according to SEQ ID No. 20 and/or,
- (ii) for absent or reduced expression of at least one gene which is essential for carotenoid synthesis, and/or,
- (iii) for overexpression of dTDP-D-glucose-4,6-dehydratase AcbB according to SEQ ID No. 13, and/or
- (iv) for overexpression of UDP-glucose-1P uridyltransferase GtaB according to SEQ ID No. 19.
An Actinomycetales strain, preferably an Actinoplanes strain, for the production of acarbose, wherein the Actinomycetales strain is genetically engineered for absent or reduced expression of extracellular small carbohydrate binding protein Cgt according to SEQ ID No. 20.
An Actinomycetales strain, preferably an Actinoplanes strain, for the production of acarbose, wherein the Actinomycetales strain is genetically engineered for absent or reduced expression of at least one gene which is essential for carotenoid synthesis.
An Actinomycetales strain, preferably an Actinoplanes strain, for the production of acarbose, wherein the Actinomycetales strain is genetically engineered for overexpression of dTDP-D-glucose-4,6-dehydratase AcbB according to SEQ ID No. 13.
An Actinomycetales strain, preferably an Actinoplanes strain, for the production of acarbose, wherein the Actinomycetales strain is genetically engineered for overexpression of UDP-glucose-1P uridyltransferase GtaB according to SEQ ID No. 19.
An expression vector for Actinoplanes comprising an expression cassette for AcbB according to SEQ ID No. 13 and/or an expression cassette for GtaB according to SEQ ID No. 19, and/or an expression cassette for MerR according to SEQ ID No. 22.
Use of an Actinomycetales strain according to any previous claim in the production of acarbose.
Dependencies are inferred from the claim text.