Mass production method of uniform size cell aggregates
AssigneesOrizuru Therapeutics, Inc., Toyo Seikan Group Holdings, Ltd.
InventorsJunji YAMAURA, Ryo ITO, Taro TOYODA, Shuhei KONAGAYA, Ryo SUENAGA
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Abstract
An object of the present invention is to provide a novel approach capable of conveniently producing a large quantity of substantially uniform size cell aggregates. Provided is a method for producing cell aggregates using a cell culture bag, the cell culture bag having a lower face comprising a plurality of recesses, the method comprising the steps of: (1) adding cells and a medium to the cell culture bag, stirring the contents, and culturing the cells while applying a pressure to the cell culture bag; and (2) recovering the formed cell aggregates after the completion of culture.
Claims26
A method for producing cell aggregates using a cell culture bag,
- the cell culture bag having an upper face and a lower face and comprising a plurality of recesses on the lower face,
- the method comprising the steps of:
- (1) adding cells and a medium to the cell culture bag, stirring the contents, and culturing the cells while applying a pressure in at least one direction to the cell culture bag;
- and
- (2) recovering the formed cell aggregates after the completion of culture.
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A cell aggregate population for transplantation, wherein 70% or more of the cell aggregates have a particle size of within ±10% of the median particle size of the cell aggregates.
A cell culture bag comprising 100000 to 1000000 cell aggregates having a substantially uniform particle size.
Dependencies are inferred from the claim text.