Buckwheat-derived c-glycosyltransferase gene and utilization thereof
AssigneeSuntory Holdings Limited
InventorsNoriko Nakamura, Naoko Okitsu, Yukihisa Katsumoto
Life of this patent
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Abstract
Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.
Claims19
A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog, wherein:
- the buckwheat-derived CGT gene or its homolog is selected from the group consisting of:
- (1-a) a polynucleotide consisting of the nucleotide sequence listed as SEQ ID NO:
- 11;
- and
- (1-b) a polynucleotide that hybridizes with a polynucleotide consisting of the nucleotide sequence complementary to the nucleotide sequence listed as SEQ ID NO:
- 11 under stringent conditions, and has the same activity as the polynucleotide of (1-a).
A vector comprising a buckwheat-derived CGT gene or its homolog, wherein the buckwheat-derived CGT gene or its homolog is selected from the group consisting of:
- (1-a) a polynucleotide consisting of the nucleotide sequence listed as SEQ ID NO:
- 11;
- (1-b) a polynucleotide that hybridizes with a polynucleotide consisting of the nucleotide sequence complementary to the nucleotide sequence listed as SEQ ID NO:
- 11 under stringent conditions and has the same activity as the polynucleotide of (1-a);
- (1-c) a polynucleotide that encodes a protein consisting of the amino acid sequence listed as SEQ ID NO:
- 12;
- (1-d) a polynucleotide that encodes a protein consisting of an amino acid sequence that is the amino acid sequence listed as SEQ ID NO:
- 12 having a deletion, substitution, insertion and/or addition of one or more amino acids, and having the same activity as a protein encoded by the polynucleotide of (1-c);
- and
- (1-e) a polynucleotide that encodes a protein having an amino acid sequence with at least 90% identity with respect to the amino acid sequence listed as SEQ ID NO:
- 12 and having the same activity as a protein encoded by the polynucleotide of (1-c).
A method for creating transgenic plants with blue flower color, comprising a step of transferring a buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to coexist in the plant cells.
Dependencies are inferred from the claim text.