Composition and method for inactivating a virus

A method for detecting an analyte in a sample, wherein the analyte is a biomolecule selected from an RNA and a DNA, the method comprising:

• preparing the sample for nucleic acid analysis by contacting the sample with a deep eutectic solvent;
• and
• performing nucleic acid analysis to detect the analyte,
• wherein the deep eutectic solvent in activates a virus in the sample, wherein the nucleic acid analysis is carried out in the presence of crude cellular components and wherein the deep eutectic solvent is trimethylglycine:trifluoroacetamide.

A method of manufacturing a vaccine for preventing a viral disease, which method comprises:

• preparing an inactivated virus by contacting a virus with a composition comprising a quaternary ammonium compound and a halogenated organic compound;
• and
• formulating the inactivated virus in a pharmaceutically-acceptable carrier, wherein the quaternary ammonium compound is a compound of Formula 1 or a salt thereof:
• wherein:
• R1, R2 and R3 are each independently selected from a methyl group and ethyl group:
• R4 is H or OH:
• R5 is selected from H, a halide, a carbonyl oxygen, a methyl group, and
• wherein:
• A1 is selected from CH2, O and S;
• R7 is selected from OH, an unsubstituted C1 to C3 alkyl group, an alkyl carboxylic acid group having 1 to 3 carbon atoms, and a substituted C1 to C3 alkyl group bearing one or more substituents selected from hydroxyl and halide groups;
• with the proviso that, when R5 is H or a methyl group, R4 is OH;
• and wherein the halogenated organic compound is a compound of Formula 2:
• wherein:
• A2 is O or S;
• R8 is a substituted C1 to C12 alkyl group bearing one or more substituents selected from fluoro, chloro, bromo, and iodo;
• and
• i) R9 and R10 are each independently selected from H, an unsubstituted C1 to C12 alkyl group:
• an unsubstituted alkyl ester group having 2 to 12 carbon atoms;
• an alkyl ester group having 2 to 12 carbon atoms and bearing one or more substituents selected from fluoro, chloro, bromo, iodo, and hydroxyl;
• and a substituted C1 to C12 alkyl group bearing one or more substituents selected from fluoro, chloro, bromo, iodo, and hydroxyl;
• or
• ii) R9 and R10 together form an imidazole group.