Growth factor composition for cell culture-produced meat
AssigneeORF Liftaekni hf.
InventorsBjorn Larus ORVAR, Arna RUNARSDOTTIR, Jon Mar BJORNSSON, Hilmar VIDARSSON
Life of this patent
Estimating the term…
Abstract
The invention relates to the use of a growth factor composition comprising one or more recombinant animal growth factor and one or more plant seed protein for cultivating cells for the production of cell culture meat. The invention also relates to a method of cultivating cells for the production of cell culture meat using a growth factor composition that comprises one or more recombinant animal growth factor and one or more plant seed protein.
Claims27
Use of a growth factor composition comprising one or more recombinant animal growth factor and one or more plant seed protein for cultivating cells for the production of cell culture meat, wherein the growth factor composition is from a non-animal source and preferably produced in a transgenic plant.
A method of cultivating cells for the production of cell culture meat, the method comprising:
- b. providing at least one cell culture that is capable of growing to generate a meat-like tissue, and
- c. supplying said at least one cell culture with a growth medium for sustaining growth and/or differentiation of said cell culture,
- characterised in that said growth medium comprises at least one growth factor composition that comprises one or more recombinant animal growth factor and one or more plant seed protein, wherein said growth factor is from a non-animal source and preferably produced in a transgenic plant.
A growth factor supplement, in particular a growth factor supplement for use in the cultivation of cells for the production of cell culture meat, the growth factor supplement comprising an extract from transgenic plant material selected from the group consisting of barley, wheat, oat, rye, maize, rice, soya, peas, millets, sorghum and rape, the extract comprising in the range from about 0.1% to about 97% by weight of one or more recombinant animal growth factor and one or more native plant seed protein selected from the group consisting of dehydrins, protease inhibitors, hordeins, late-embryogenesis abundant proteins, (LEA), cyclophilins, ABA-responsive proteins, globulins, albumins, prolamins, vicilins, glutelins, and zeins.
A method for producing a growth factor supplement in particular for supplementing cell culture medium for cell culture meat production, the method comprising
- providing a plant expressing a transgenic animal growth factor in its seeds and growing said plant until obtaining mature seeds,
- harvesting said seeds,
- cleaning the seeds with seed cleaning or seed sorting machine,
- de-hulling the cleaned or sorted seeds,
- sterilizing the de-hulled seeds and milling to fine powder,
- extracting soluble proteins in extraction buffer,
- clarifying the obtained extract,
- optionally further purifying said growth factor from the extract.
Dependencies are inferred from the claim text.