Culture medium for laryngeal cancer epithelial cells, culture method, and application thereof
AssigneeCas (Hefei) Institute of Technology Innovation
InventorsQing Song LIU, Jie HU, Tao REN
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Abstract
Provide are a primay cell culture medium that contains an MST1/2 kinase inhibitor and is used for culturing laryngeal cancer epithelial cells, and a culture method using the primary cell culture medium. In the culture method, the primay cell culture medium is used to culture primary cells on a clulture vessel plated with irradiated trophoblasts, so that the primay cells proliferate rapidly. A cell model obtained by the primary cell culture medium and the primay cell culture method can be used for the efficacy evaluation and screening of drugs.
Claims14
A primary cell culture medium for culturing laryngeal cancer epithelial cells:
- comprising an MST1/2 kinase inhibitor, wherein the MST1/2 kinase inhibitor comprises a compound of Formula (I) or a pharmaceutically acceptable salt, or a solvate thereof,
- wherein,
- R1 is selected from C1-C6 alkyl, C3-C6 cycloalkyl, C4-C8 cycloalkylalkyl, C2-C6 spirocycloalkyl, and aryl optionally independently substituted with 1-2 R6, aryl C1-C6 alkyl optionally independently substituted with 1-2 R6 and heteroaryl optionally independently substituted with 1-2 R6;
- R2 and R3 are each independently selected from C1-C6 alkyl;
- R4 and R5 are each independently selected from hydrogen, C1-C6 alkyl, C3-C6 cycloalkyl, C4-C8 cycloalkylalkyl, hydroxyl C1-C6 alkyl, C1-C6 haloalkyl, C1-C6 alkylamino C1-C6 alkyl, C1-C6 alkoxy C1-C6 alkyl, and C3-C6 heterocyclyl C1-C6 alkyl;
- and
- R6 is selected from halogen, C1-C6 alkyl, C1-C6 alkoxy, and C1-C6 haloalkyl.
Dependencies are inferred from the claim text.