De novo synthesized gene libraries
AssigneeTwist Bioscience Corporation
InventorsWilliam BANYAI, Bill James PECK, Andres FERNANDEZ, Siyuan CHEN, Pierre INDERMUHLE
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Abstract
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
Claims21
1-20. (canceled)
A system for constructing a nucleic acid molecule comprising an identifier, the system comprising:
- (a) a first printhead configured to deposit a first component of the nucleic acid molecule comprising an identifier on a specified site on a substrate;
- (b) a second printhead configured to deposit a second component of the nucleic acid molecule comprising an identifier on the specified site on the substrate;
- and
- (c) a dispenser configured to deposit a reaction mix onto the specified site on the substrate to link the first component and the second component to construct the nucleic acid molecule,
- wherein the nucleic acid molecule comprising the identifier represents sequence information.
A method for constructing a nucleic acid molecule comprising an identifier, the method comprising:
- (a) depositing a first droplet comprising a first component of the nucleic acid molecule comprising an identifier on a specified site on a substrate using a first printhead;
- (b) depositing a second droplet comprising a second component of the nucleic acid molecule comprising an identifier on the specified site on a substrate using a second printhead;
- and
- (c) linking the first component and the second component to construct the nucleic acid molecule using a dispenser, wherein the linking is performed by dispending a reaction mix onto the specified site on the substrate, providing a condition necessary to link the first component and the second component, or both,
- wherein the nucleic acid molecule comprising the identifier represents sequence information.
Dependencies are inferred from the claim text.