Nanosatellite-substrate complex and method of regulating stem cell adhesion and differentiation using the same
AssigneeKorea University Research and Business Foundation
InventorsYoung-Keun KIM, Heemin KANG, THOMAS MYEONGSEOK KOO, Myeong-Soo KIM, Sung-Gue LEE, Gun-Hyu BAE
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Abstract
The present invention relates to a nanosatellite-substrate complex capable of regulating stem cell adhesion and differentiation, and a method for preparing the same. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation by applying a magnetic field to the nanosatellite-substrate complex.
Claims20
A nanosatellite-substrate complex for regulating stem cell adhesion and differentiation comprising:
- a substrate;
- a core-shell-type magnetic particle provided to be spaced apart from at least one side of the substrate;
- a gold nanoparticle connected to one side of the magnetic particle;
- a first linker connecting between the substrate and the gold nanoparticle;
- a second linker connecting between the gold nanoparticle and a ligand;
- and
- the ligand connected to the gold nanoparticles via the second linker,
- wherein the gold nanoparticle is connected to the ligand via the second linker to form a nanoassembly,
- the magnetic particle is conjugated to the nanoassembly to form a nanosatellite structure,
- the nanoassembly comprises one or more gold nanoparticles, one or more second linkers connected to at least one of the one or more gold nanoparticles, and the ligand connected to the second linker, and
- one or more nanoassemblies are comprised in the nanosatellite structure.
A method for preparing a nanosatellite-substrate complex for regulating stem cell adhesion and differentiation, the method comprising:
- coating a surface of iron oxide with a silica having an amine group to form magnetic particles;
- providing gold nanoparticles on surfaces of the magnetic particles;
- adding and dispersing the magnetic particles having the gold nanoparticles provided thereon in a solution containing a polymer linker to form a first linker and a second linker;
- reacting the first linker with a substrate having amine groups formed thereon, so that the first linker is bound to at least a portion of the amine groups formed on the substrate and the magnetic particles having the gold nanoparticles provided thereon are conjugated to the substrate;
- deactivating amine groups, which remain unbound to the first linker on the substrate, by treatment with a deactivating group;
- and
- conjugating a ligand to the second linker.
Dependencies are inferred from the claim text.