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AssigneeThe Johns Hopkins University
InventorsRachel A. Hegab, Brooke M. Luisi, Julie E. Gleason
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Provided is a whole-cell biosensor system with robust biocontainment for field deployment and a strong visual reporter for readouts in the deployed environment. The engineered biosensors in an encapsulated and deployable system (eBEADS) demonstrate a portable, no power living sensor for detection of environmental pollutants, e.g., 2-phenylphenol (2-PP). The whole-cell biosensor system uses bacteria engineered to detect an analyte and generate a visual colorimetric output upon being contacted with the analyte. Advantageously, the analyte is detectable with the naked eye and the whole-cell biosensor system enables analyte detection without electronics.
A polynucleotide comprising a Pseudomonas azelaica hbpR (hbpR) promoter operably linked to a polynucleotide encoding a HbpR protein and a heterologous promoter operably linked to a polynucleotide encoding a marker protein, wherein the marker protein creates a visible output signal.
A polynucleotide comprising:
A method of preparing a biosensor system, the method comprising:
Dependencies are inferred from the claim text.