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AssigneeSelexis S.A.
InventorsLucille Pourcel, Audrey Berger, Valerie Le Fourn, Severine Fagete, David Calabrese, Alexandre Regamey, Nicolas Mermod, Fabien Palazzoli, Pierre-Alain Girod
Estimating the term…
A transcriptomic analysis of genes consistently upregulated in high producer clones were each evaluated for their ability to increase the production of a protein of interest. The products of these genes (metabolism influencing products (MIP)), such as actin, Erp27, Erp57, Foxa1, PPAR, Ca3, and Tagap, could be sub-categorized into different functional categories such as signaling, protein folding, cytoskeleton organization and cell survival.
A eukaryotic expression system comprising:
A recombinant eukaryotic cell comprising at least one endogenous MIP under the control of at least one exogenous promoter selected from the group of CMV, EF1alpha, CMV/EF1 alpha, SV40, RSV, PGK, a exogenous or recombinant endogenous promoter having an expression level of CMV, EF1alpha, CMV/EF1alpha, SV40, RSV, PGK and combinations thereof.
(canceled)
Dependencies are inferred from the claim text.