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AssigneeRiver Stone Biotech, LLC
InventorsMarkus Schwab, Franziska Grassinger, Laura Occhipinti, Philipp Friedrich Berninger, Jon Richard Heal, Joseph Michael Sheridan, Anaelle Hatsch, Jens Houghton-Larsen
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The invention relates to norcoclaurine synthases and substrate binding sites having one or more site-specific mutation which increase the activity, when compared to the wild type synthase, of the condensation of 4-HPAA and dopamine to (S)-norcoclaurine and/or 3,4-DhPAA and dopamine to (S)-norlaudanosoline. The inventors both identified specific mutations corresponding to at position 73, 75, 77, 82, 99, 114, 141, 142, 147, 152, 174 and/or 178 in the count according to SEQ ID No: 1, and sites corresponding to the binding domains defined in SEQ ID NO: 4 and 5, where the mutated increase of the activity may be positioned within these norcoclaurine synthases. These domains are conserved regions.
A norcoclaurine synthase comprising an amino acid sequence which is at least 40% identical to the amino acid sequence given in SEQ ID No. 1, and which has one or more site-specific mutations corresponding to position 73, 75, 77, 82, 99, 114, 141, 142, 147, 152, 174 and/or 178 of SEQ ID No. 1.
Dependencies are inferred from the claim text.