Compositions and Methods for Recombinant Biosynthesis of Cannabinoids
AssigneeWillow Biosciences, Inc.
InventorMathias Schuetz
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Abstract
The present disclosure provides recombinant host cells comprising a pathway capable of producing a cannabinoid and a nucleic acid derived from a Cannabis trichome mRNA that that does not encode an enzyme in the pathway but enhances the host cells' ability to produce the cannabinoid. The disclosure also provides methods of using host cells to produce cannabinoids.
Claims17
A recombinant host cell comprising a pathway capable of producing a cannabinoid and a nucleic acid derived from a Cannabis trichome mRNA that does not encode an enzyme in the pathway, wherein the nucleic acid derived from a Cannabis trichome mRNA encodes a polypeptide having a protein function selected from:
- lipid transfer type protein, mulatexin-like, amino acid biosynthesis, amino acid degradation, carbohydrate metabolism, carrier-mediated transport, cell cycle organization, cellular respiration, chromatin organization, circadian clock system regulation, coenzyme metabolism, oxidoreductase, transferase, hydrolase, lyase, effector-triggered immunity network co-regulatory protein, fatty acid biosynthesis, lipid degradation, messenger ribonucleoparticle (mRNP) export, organelle machinery ribonuclease activity, phytohormone action, protein folding, protein phosphorylation, protein S-glutathionylation, protein translocation, redox homeostasis, RNA biosynthesis, RNA processing, solute transport, terpenoid metabolism, and/or vesicle trafficking.
A method for making a recombinant host cell for producing a cannabinoid comprising introducing into a host cell:
(a) a first set of nucleic acids that encode a pathway of enzymes capable of producing a cannabinoid;
- and
- 12. (b) a nucleic acid derived from a Cannabis trichome mRNA that does not encode an enzyme in the pathway, wherein the nucleic acid encodes a polypeptide having a protein function selected from:
- lipid transfer type protein, mulatexin-like, amino acid biosynthesis, amino acid degradation, carbohydrate metabolism, carrier-mediated transport, cell cycle organization, cellular respiration, chromatin organization, circadian clock system regulation, coenzyme metabolism, oxidoreductase, transferase, hydrolase, lyase, effector-triggered immunity network co-regulatory protein, fatty acid biosynthesis, lipid degradation, messenger ribonucleoparticle (mRNP) export, organelle machinery ribonuclease activity, phytohormone action, protein folding, protein phosphorylation, protein S-glutathionylation, protein translocation, redox homeostasis, RNA biosynthesis, RNA processing, solute transport, terpenoid metabolism, and/or vesicle trafficking.
The method of any one of claims 30-37, wherein the recombinant host cell is a cell derived from a source selected from:
- Saccharomyces cerevisiae, Escherichia coli, Yarrowia lipolytica, and Pichia pastoris.
Dependencies are inferred from the claim text.