US 2023/0193218 A1 · USE OF LYTIC POLYSACCHARIDE MONOOXYGENASES, ENZYMATIC COMPOSITION CONTAINING SAME, AND DEGRADATION METHOD FOR PLASTIC POLYMERS

Abstract

The present disclosure relates to the novel activity of the enzymatic composition containing lytic polysaccharide monooxygenases (LPMOs) that are bacterial (Auxiliary Activity 10, AA10) and/or fungal (Auxiliary Activity 9, AA9) for degrading polyethylene terephthalate (PET) and related plastic polymers. The genes that encode KpLPMO10A (AA10) and AfLPMO9A (AA9) were isolated from Kitasatospora papulosa and Aspergillus fischeri microorganisms, respectively. Methods such as atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) detected alterations in the superficial chemical composition and morphology of the PET found in liquid bottles when treated with LPMOs. The gentle temperature conditions used during the LPMO-PET reaction suit the use of these enzymes to help canonical enzymes (PETases) deconstruct plastics, which is beneficial for the circular economy for PET.

Claims31

Dependent claims

An enzymatic composition for use in the degradation of polyester, comprising:

at least one lytic polysaccharide monooxygenase selected from an enzyme comprising the amino acid sequence having at least 60% identity with SEQ ID NO:
2, an enzyme comprising the amino acid sequence having at least 60% identity with SEQ ID NO:
5, or a mixture thereof;
and
a vehicle.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/926,513Filed 17 May 2021

Priority

BR 102020009936-118 May 2020

Published

22 Jun 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C08J 11/105
By chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation: by treatment with enzymes
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen

Use of lytic polysaccharide monooxygenases, enzymatic composition containing same, and degradation method for plastic polymers

Life of this patent

Use of lytic polysaccharide monooxygenases, enzymatic composition containing same, and degradation method for plastic polymers

Life of this patent

Abstract

Claims31