Improved method for the production of high levels of pufa in plants
AssigneeBASF Plant Science GmbH
InventorsToralf Senger, Hui Yang, Carl Andre, Patricia Vrinten
Life of this patent
Estimating the term…
Abstract
The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).
Claims45
Raw plant seed oil comprising
- i. a level of the 18:2 fatty acid fraction in % (w/w) in the triacylglycerol (TAG) composition higher than the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction,
- ii. a level of the 20:0 fatty acid in % (w/w) in the diacylglyerol fraction higher than the 20:0 fatty acid level in % (w/w) in the triacylglycerol fraction, and/or
- iii. a level of the 22:1 fatty acid in % (w/w) in the diacylglyerol (DAG) fraction is higher than the SDA level in % (w/w) in the triacylglycerol fraction.
(canceled)
Method for increasing the delta 6 elongase conversion efficiency in oil crop plant that produces vlcPUFA and expresses a delta-6 elongase, comprising decreasing, compared to a control, in the oil crop plant, plant cell, plant seed, or a part thereof, the activity of one or more PDCT selected from the group consisting of:
- (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;
- (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59;
- (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i);
- (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity;
- (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code;
- and
- (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
An isolated, a synthetic, or a recombinant polynucleotide:
- (a) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59, wherein the nucleic acid encodes a polypeptide having PDCT3 and/or 5 activity;
- (b) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, wherein the polypeptide has PDCT3 and/or 5 activity;
- (c) a fragment of (a) or (b), wherein the fragment encodes a polypeptide having PDCT3 and/or 5 activity;
- or
- (d) a nucleic acid sequence fully complementary to any of (a) to (c),
- (f) antisense, ribozyme, microRNA polynucleotide comprising a fragment of the PDCT3 and/or PDCT5 polynucleotide of (a), (b), (c), or (d) that binds specifically to the polynucleotide of (a), (b), (c), or (d)
- and having PDCT3 and/or PDCT5 expression inhibiting activity.
(canceled)
A polypeptide selected from the group consisting of:
- (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;
- (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59;
- (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i);
- (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity;
- (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code;
- and
- (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.
An antisenseRNA, ribozyme, or microRNA comprising
- (1) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59;
- (2) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;
- (3) a fragment of (1) or (2);
- or
- (4) a nucleic acid sequence fully complementary to any of (1) to (3), whereby the antisense RNA, ribozyme, or microRNA, when expressed in a plant, plant cell or plant seed that comprises
- (i) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59, wherein the nucleic acid encodes a polypeptide having PDCT3 and/or 5 activity;
- (ii) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, wherein the polypeptide has PDCT3 and/or 5 activity;
- (ii) a fragment of (i) or (ii), wherein the fragment encodes a polypeptide having PDCT3 and/or 5 activity;
- or expresses a PDCT, wherein the PDCT is selected from the group consisting of:
- (a) a PDCT3 and/or PDCT5 having at least 90% sequence identity with SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;
- (b) a PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59;
- (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i);
- (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity;
- and
- (e) a PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60 due to the degeneracy of the genetic code,
- and reduces the expression of said nucleic acid sequence or said PDCT3 and/or 5.
(canceled)
(canceled)
(canceled)
(canceled)
A plant, or part thereof, a plant seed, a plant cell, or a plant oil comprising C18 to C22 fatty acids, wherein the ALA+LA level is less than C18 to C22 fatty acids having an increased PDCT1 and/or PDCT19 expression or acitivity.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.