New swim up method for sperm washing

A sperm wash device comprising:

• a first chamber section for holding an unwashed semen sample;
• a second chamber section for holding sperm wash media disposed over and attached to the first chamber section;
• a ledge separating the first chamber section from the second chamber section, the ledge having a slot therein that is configured be filled with sperm wash media that does not get recovered;
• and
• a slider is placed on the ledge, the slider being moveable between a first position and a second position, wherein at the first position, the slider allows fluid communication between the first chamber section and the second chamber section and at the second position, the slider blocks fluid communication between the first chamber section and the second chamber section, and
• wherein during operation, the unwashed semen sample is placed in the first chamber section with the slider at the first position and then the slider is positioned in the second position, the sperm wash media is introduced into the second chamber section, after the sperm wash media is added, the slider is repositioned in the first position thereby allowing sperm to travel from the first chamber section to the second chamber section.