Biosynthesis of mogrosides
AssigneeGinkgo Bioworks, Inc.
InventorsJeffrey Ian Boucher, Justin Michael Gardin, Scott Marr, Matthew McMahon, Krishnaben S. Patel, Jie Zhu
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Abstract
Described in this application are UDP-glycosyltransferases (UGT) enzymes, host cells expressing the UGTs, and methods of producing mogrol precursors, mogrol, and/or mogrosides using such host cells.
Claims53
A host cell that comprises a heterologous polynucleotide encoding a UDP-glycosyltransferase (UGT), wherein the UGT comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 76, 101, 89, 71, 73, 97, 88, 85, 95, 96, 86, 92, 69, 66, 78, 75, 98, 90, 67, 91, 93, 65, 99, 68, 70, 72, 81, 83, 84, 94 and 100, and wherein the UGT is capable of catalyzing conversion of mogrol to MIA1.
A host cell that comprises a heterologous polynucleotide encoding a UDP-glycosyltransferase (UGT), wherein the UGT comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 103, 74, 79, 102, 62, and 87 and wherein the UGT is capable of catalyzing conversion of mogrol to MIE1.
A host cell that comprises a heterologous polynucleotide encoding a UDP-glycosyltransferase (UGT), wherein the UGT comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 63, 77, 84, and 94, and wherein the UGT is capable of catalyzing conversion of mogrol to MIIE1.
A host cell that comprises a heterologous polynucleotide encoding a UDP-glycosyltransferase (UGT), wherein the UGT comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 104-106 and 108-114, and wherein the UGT is capable of producing one or more mogrosides from a mogroside precursor.
Dependencies are inferred from the claim text.