Method for Preparing Combinatorial Library of Multi-Modular Biosynthetic Enzyme Gene
AssigneeSpiber Inc.
InventorsDavid Lips, Gijs Verkleij
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method including (A) a step of preparing a plurality of DNA fragments which are capable of reconstructing the gene cluster construct and have structures that can be ligated to each other and (B) a step of ligating the plurality of DNA fragments prepared in the step (A) to each other by mixing the plurality of DNA fragments in a solution to obtain the gene cluster construct.
Claims11
A method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method comprising:
- (A) a step of preparing a plurality of DNA fragments which are capable of reconstructing the gene cluster construct and have structures that can be ligated to each other;
- and
- (B) a step of ligating the plurality of DNA fragments prepared in the step (A) to each other by mixing the plurality of DNA fragments in a solution to obtain the gene cluster construct.
A method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method comprising the following steps:
- (P) a step of preparing a plurality of types of plasmids containing a plurality of DNA fragments having structures that can be ligated to each other;
- (A1) a step of preparing a mixture solution of the plurality of types of DNA fragments by treating the plurality of types of plasmids with restriction enzymes suitable to each of the plasmids;
- and
- (B1) a step of reaccumulating the DNA fragments using the mixture solution of the DNA fragments obtained in the step (A1) to obtain the gene cluster construct.
Dependencies are inferred from the claim text.