Recombinant bacteria for production of indole-3-acetic acid (iaa) and uses thereof

The bacterium of any one of the previous claims, further comprising a second gene cassette, wherein the second gene cassette comprises one or more sequences encoding a protein selected from the group consisting of AroG, TrpDH, IpdC, Iad1, and a combination thereof.

The bacterium of claim any one of the previous claims, wherein the bacterium further comprises a deletion or a mutation in a gene encoding tryptophan transcriptional repressor (TrpR) and/or a deletion or a mutation in a gene encoding tryptophanase (TnaA).

The bacterium of any one of the previous claims, wherein the promoter is directly or indirectly induced by low-oxygen or anaerobic conditions.

The bacterium of any one of the previous claims, wherein the promoter is an FNR-inducible promoter.

The bacterium of any one of the previous claims, wherein the one or more gene cassettes and operatively linked promoter are present on a plasmid in the bacterium.

The bacterium of any one of the previous claims, wherein the bacterium is a non-pathogenic bacterium.

The bacterium of any one of the previous claims, wherein the bacterium is a probiotic or a commensal bacterium.

The bacterium of claim any one of the previous claims, wherein the bacterium is selected from the group consisting of Bacteroides, Bifidobacterium, Clostridium, Escherichia, Lactobacillus, and Lactococcus.

The bacterium of any one of the previous claims, wherein the bacterium is capable of producing about 1 μM IAA to about 200 μM IAA.

The bacterium of any one of the previous claims, wherein the bacterium is capable of producing about 1 μM IAA to about 200 μM tryptophan.

A pharmaceutically acceptable composition comprising the bacterium of any one of the previous claims and a pharmaceutically acceptable carrier.