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US 2023/0172222 A1Published application
Redefining livestock milk by removing folate receptor protein to decrease folate receptor autoimmune disorder in pregnancy and autism
AssigneeMiritaur LLC
InventorsPrem Premsrirut, Edward V. Quadros
Life of this patent
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Abstract
Provided herein are systems and methods in Redefining Cow Milk to decrease the prevalence of folate receptor autoimmune disorder in the population to reduce the Incidence of neural tube defect pregnancy, pre-term birth, subfertility and a plethora of developmental disorders including autism spectrum disorders.
Claims16
A method to produce folate receptor free (FRF) milk comprising:
- removing folate receptor from a milk product using a method selected from the group consisting of:
- size exclusion chromatography, Glycophosphate inositol (GPI) capture column affinity chromatography, Folate Receptor binding antibody affinity column chromatography, specific digestion of the folate receptor using proteases attached to the FR binding antibody, Fractionation of the milk products to remove the FR, or Affinity chromatography using ligands that specifically bind the folate receptor in milk.
A system for folate receptor free milk to decrease rates of Autism, comprising:
- generating shRNAs targeting the folate receptor in a mammal, conducting nucleofection of the shRNAs, identifying the positive single clone, and conducting nuclear transfer into a mammal producing milk lacking folate receptor antigens.
A Knockdown Folate Receptor in cow fibroblast method comprising:
- identifying shRNA's in a shRNA library and validating the shRNA's in a sensor assay to find shRNAs that potently and specifically silence the folate receptor;
- confirming the shRNA knockdown in a fibroblast;
- cloning a vector containing an shRNA or concatemer of shRNAs;
- and inserting the vector into fibroblasts.
1 more element
A milk product, comprising:
- a folate free milk product used to make a product selected from the group consisting of:
- bulk milk, bulk milk used to make cheese whether or not the milk has been pasteurized, sterilized or otherwise treated to reduce the population of microbes prior to cheese making, milk powders, milk solids, caseins, caseinates, and casein hydrolysates, pasteurized, sterilized, preserved milks including microfiltered milks, UHT milks, low fat milks, modified or enhanced milks, ice-cream or other frozen dairy based confections, fermented milk products such as yoghurt or quark, cheeses including full fat, partial de-fatted and fat-free processed cheeses, milk whey, food products enriched through the addition of milk products such as soups, milk from which potentially allergenic molecules have been removed, confections such as chocolate, carbonated milk products, including those with added phosphate and/or citrate, infant formulations which may contain full, partially de-fatted or nonfat milk together with a number of additional supplements, liquid or powdered drink mixtures, and buttermilk and buttermilk powder.
Dependencies are inferred from the claim text.