Method for detecting tyrosine residue in sample, and terbium compound to be used for such detection
AssigneeNEC Corporation
InventorsKatsumi MAEDA, Masayoshi TSUJI
Life of this patent
Estimating the term…
Abstract
An example objective of the present invention is to provide a method for detecting a tyrosine residue in a sample and a compound that can be used in the method. One aspect of the present embodiment relates to a method for detecting a tyrosine residue in a sample, wherein fluorescence emission from a reaction product of a terbium compound represented by the predetermined formula and a tyrosine residue is detected.
Claims7
A method for detecting a tyrosine residue in a sample, wherein fluorescence emission from a reaction product of a terbium compound and a tyrosine residue is detected, the terbium compound being represented by the following formula (1):
- wherein R groups each independently represent alkyl group having 1 to 8 carbon atoms, alkenyl group having 2 to 8 carbon atoms, cycloalkyl group having 3 to 8 carbon atoms, or substituted or unsubstituted aryl group;
- R groups attached to the same X atom or different X atoms are optionally attached to each other;
- X represents phosphorus atom or sulfur atom;
- Y represents hydrocarbon group having 1 to 12 carbon atoms;
- n represents an integer of 1 to 3 and m represents an integer of 0 to 5;
- and, if m is 2 or more, X atoms may be identical to or different from each other.
A terbium compound represented by the following formula (2):
- wherein R groups each independently represent alkyl group having 1 to 8 carbon atoms, alkenyl group having 2 to 8 carbon atoms, cycloalkyl group having 3 to 8 carbon atoms, or substituted or unsubstituted aryl group;
- R groups attached to the same X atom or different X atoms are optionally attached to each other;
- X represents phosphorus atom or sulfur atom;
- Y represents hydrocarbon group having 1 to 12 carbon atoms;
- n represents an integer of 1 to 3 and m represents an integer of 1 to 5;
- and, if m is 2 or more, X atoms may be identical to or different from each other.
A sensor for detecting a tyrosine residue, the sensor comprising a detection section configured to detect fluorescence emission from a reaction product of a terbium compound and a tyrosine residue, the terbium compound being represented by the following formula (1):
- wherein R groups each independently represent alkyl group having 1 to 8 carbon atoms, alkenyl group having 2 to 8 carbon atoms, cycloalkyl group having 3 to 8 carbon atoms, or substituted or unsubstituted aryl group;
- R groups attached to the same X atom or different X atoms are optionally attached to each other;
- X represents phosphorus atom or sulfur atom;
- Y represents hydrocarbon group having 1 to 12 carbon atoms;
- n represents an integer of 1 to 3 and m represents an integer of 0 to 5;
- and, if3 m is 2 or more, X atoms may be identical to or different from each other.
Dependencies are inferred from the claim text.