Depletion of ext1 expression and/or activity improves cellular production of biological entities
AssigneeUniversite De Liege
InventorsJean-Claude TWIZERE, Despoina KERSELIDOU
Life of this patent
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Abstract
The use of an inhibitor of EXT1 expression and/or activity for the production of a biological entity in a cell. Also, the use of a cell having at least depleted EXT1 expression and/or activity for the production of a biological entity. Further, evidence is provided about the role of glycosylation in rapid dynamism of ER shaping and function. In particular, depletion of EXT1 results in a recomposed ER shaping, which could benefit production of recombinant proteins.
Claims20
1-15. (canceled)
A method for the production of a biological entity in a cell, said method comprising the steps of:
- a) providing a cell population having at least depleted EXT1 expression and/or activity;
- b) transfecting the cell population of step a) with an oligonucleotide encoding the biological entity, preferably a polypeptide or a viral particle.
A method for the production of a biological entity in a cell, said method comprising the steps of:
- a) providing a cell population;
- b) transfecting the cell population of step a) with an oligonucleotide encoding the biological entity.
- c) inhibiting EXT1 expression in the said cell by using an inhibitor of EXT1 expression and/or activity.
Dependencies are inferred from the claim text.