Screening of Engineered Biocatalysts for Oxyfunctionalization of Olefins
OwnerXiaozhou Zhang, Mohor Chatterjeeinventors
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Abstract
A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.
Claims20
A screening method for identifying engineered biocatalysts, comprising:
- reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons;
- reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons;
- and
- measuring activity of the engineered biocatalyst.
A screening system for identifying biocatalysts, comprising:
- one or more recombinant plasmids configured to express an engineered biocatalyst that oxyfunctionalizes olefins and a fatty alcohol oxidase that oxidizes fatty alcohols;
- and
- a hydrogen peroxide assay.
Dependencies are inferred from the claim text.