Preparation and use of plant embryo explants for transformation
AssigneeMonsanto Technology LLC
InventorsBeth Jo Calabotta, Kevin Lee Deppermann, Erik D. Dersch, Jerald D. Heise, Angela Ranae Koestel, Cindy L. Ludwig, Brian J. Martinell, Edward Williams
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Abstract
The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
Claims42
(canceled)
The explant of claim 41, wherein the explant comprises a substantially intact seed modified to expose at least a first transformable cell of the seed.
The explant of claim 41, wherein preparing the explant is carried out in the absence of a liquid.
The explant of claim 41, wherein the seed or explant comprises an internal moisture content of from about 3% to about 25%.
The explant of claim 41, wherein the method is further defined as comprising preparing a plurality of explants from a plurality of seeds and selecting one or more explant exhibiting at least a first characteristic indicative of the ability to transform the explant and/or regenerate a plant from the explant.
The explant of claim 41, wherein the plant seed is a soybean, canola, corn, cotton, onion, pepper, tomato, cucurbit, rice, or wheat seed.
The explant of claim 41, wherein preparing the explant is automated.
A regenerable explant competent for genetic transformation produced by a method comprising:
- (a) obtaining an explant from a plant seed;
- and
- (b) dehydrating the plant seed and/or explant prior to, concurrently with or after said step (a) to obtain an explant that does not germinate and remains viable and competent for genetic transformation.
A regenerable explant competent for genetic transformation produced by a method comprising the steps of:
- (a) obtaining a plant seed;
- and
- (b) preparing a regenerable explant from the plant seed under conditions wherein the explant does not germinate and remains viable and competent for genetic transformation.
-45. (canceled)
Dependencies are inferred from the claim text.