A method for providing a vlp derived from john cunningham virus
AssigneeNEUWAY Pharma GmbH
InventorsVictoria DEMINA, Marcus STAFF
Life of this patent
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Abstract
The invention relates to a method for providing a virus-like particle (VLP) derived from John Cunningham virus (JCV), relates to a VLP associated with a cargo, in particular a protein, and a drug delivery system (DDS) obtainable by said method, in particular for crossing the blood brain barrier (BBB), and a VLP containing composition. The method comprises steps of disassembly of VLP into pentamers, inducing the pentamers to aggregate and reassembly into VLP.
Claims30
Method for providing a virus-like particle (VLP) derived from John Cunningham virus (JCV) comprising the following steps:
- a) providing a composition comprising VP1 proteins,
- b) exposing the VP1 proteins of the composition of a) to conditions inducing the VP1 to assemble into VLP,
- c) exposing the VLP of the composition of b) to conditions disassembling the VLP into pentamers,
- d) exposing the pentamers of the composition of c) to conditions inducing the pentamers to reassemble into VLP
- c) exposing the VLP of the composition of d) to conditions disassembling the VLP into pentamers,
- f) exposing the pentamers of the composition of e) to conditions inducing the aggregation of the pentamers,
- g) exposing the aggregated pentamers of the composition of f) to a cargo, in particular a protein, under conditions inducing the aggregated pentamers to assemble into a VLP associated with the cargo, in particular the protein.
Composition comprising virus-like particles (VLP) derived from John Cunningham virus (JCV) characterized by one or more of the following parameters:
- a) a polydispersity index (PDI) of less than 0.3, preferably less than 0.2, preferably less than 0.1, more preferably in a range between 0.01 and 0.09,
- b) at least 70% of VLP with an average diameter from 20 nm to 70 nm, preferably of 30 nm to 70 nm, more preferably of 35 nm to 65 nm, more preferably of 40 to 60 nm,
- c) a VLP content within the composition of at least 80% (v/v), preferably at least 85% (v/v), preferably at least 90% (v/v), preferably at least 95% (v/v).
Dependencies are inferred from the claim text.