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US 2023/0148587 A1Published application
Preservation of natural and bioengineered tissues and methods of storing and transport
AssigneeTissue Testing Technologies LLC
InventorsLia H. CAMPBELL, Kelvin G.M. BROCKBANK
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Abstract
Described herein are methods for preserving biomaterials by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods. Also described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample).
Claims23
A method for preserving at least one bioengineered construct or natural tissue sample, comprising:
- (i) immersing the at least one bioengineered construct or natural tissue sample in a series of solutions having increasing concentrations of cryoprotectant to form at least one first bioengineered construct or natural tissue that is immersed in a final solution with a cryoprotectant concentration of less than or equal to 70% by weight;
- (ii) cooling the at least one first bioengineered construct or natural tissue in the final solution having said cryoprotectant concentration of less than or equal to 70% by weight to a temperature below the glass transition temperature of the final solution having said cryoprotectant concentration of less than or equal to 70% by weight;
- and
- (iii) immersing the at least one first bioengineered construct or natural tissue in a series of solutions having decreasing concentrations of cryoprotectant to obtain at least one second bioengineered construct or natural tissue immersed in a substantially cryoprotectant-free solution, the at least one second bioengineered construct or natural tissue being a substantially cryoprotectant-free construct.
1 more element
The method of claim 14, wherein the cryoprotectant concentration of the final solution of the step (ii) is in the range of from 8.0 to 11.0 M.
Dependencies are inferred from the claim text.