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US 2023/0147733 A1Published application
Oxidized tumor cell lysates encapsulated in liposomal spherical nucleic acids as potent cancer immunotherapeutics
AssigneeNorthwestern University
InventorsChad A. Mirkin, Cassandra Elizabeth Callmann
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Abstract
The disclosure is generally related to nanoparticles have an oxidized tumor cell lysate encapsulated therein and oligonucleotides on the surface thereof. Methods of making and using the nanoparticles are also provided herein.
Claims57
A nanoparticle having a substantially spherical geometry comprising an oligonucleotide conjugated thereto, wherein the oligonucleotide is a Toll-Like Receptor (TLR) agonist, and wherein an oxidized tumor cell lysate is encapsulated within the nanoparticle.
A method of making a liposomal nanoparticle comprising:
- exposing a tumor cell to an oxidizing agent to produce an oxidized tumor cell;
- then
- isolating lysate from the oxidized tumor cell;
- then
- contacting a lipid film with the lysate to produce a small unilamellar vesicle (SUV) comprising the lysate encapsulated therein;
- then
- adding an oligonucleotide to the SUV to make the liposomal nanoparticle.
4 more elements
Dependencies are inferred from the claim text.