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US 2023/0147253 A1Published application
Platelet production method and device
AssigneeKyoto University
InventorsKoji ETO, Sou NAKAMURA, Shinya SAKUMA, Hiroki KUMON, Fumihito ARAI, Yusuke KASAI
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Abstract
The present invention provides a method for producing a platelet comprising: (a) a step of culturing a megakaryocyte for at least 6 days in a platelet production medium in which a turbulent flow is generated; and (b) a step of injecting the medium comprising the megakaryocyte that has undergone step (a) into a predetermined platelet production device to expose the megakaryocyte to a laminar flow.
Claims9
A method for producing a platelet, comprising the steps of:
- (a) culturing a megakaryocyte for at least 6 days in a platelet production medium in which a turbulent flow is generated;
- and
- (b) injecting the medium comprising the megakaryocyte that has undergone step (a) into a platelet production device to expose the megakaryocyte to a laminar flow, wherein the platelet production device comprises
- an injection port for a megakaryocyte,
- a platelet collection section, and
- a channel extending from the injection port to the collection section, the channel is configured such that
- a height of an end of the channel on the injection port side is greater than a maximum diameter of a megakaryocyte to be injected,
- a height of an end of the channel on the collection section side is less than a minimum diameter of a megakaryocyte to be cell injected and greater than a maximum diameter of a platelet, and
- the height of the channel decreases from the injection port toward the collection section,
- and thereby the platelet production device is configured to make it possible to expose the megakaryocyte to the laminar flow in a state in which the megakaryocyte is captured in the channel and make it possible to release a platelet produced by the megakaryocyte from the channel into the collection section.
7 more elements
A platelet production device comprising:
- an injection port for a megakaryocyte;
- a platelet collection section;
- and
- a channel extending from the injection port to the collection section, wherein the channel is configured such that
- a height of an end of the channel on the injection port side is greater than a maximum diameter of a megakaryocyte to be injected,
- a height of an end of the channel on the collection section side is smaller than a minimum diameter of a megakaryocyte injected and greater than a maximum diameter of a platelet, and
- the height of the channel decreases from the injection port toward the collection section, and
- thereby, the platelet production device is configured to make it possible to expose the megakaryocyte to a laminar flow in a state in which the megakaryocyte is captured in the channel, and make it possible to release a platelet produced by the megakaryocyte from the channel into the collection section.
5 more elements
Dependencies are inferred from the claim text.