Liquid handling device
AssigneeOsler Diagnostics Limited
InventorsBarry Lillis, Steven Green, Vincent Linden, Konstantinos Psarros
Life of this patent
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Abstract
The present disclosure relates to a liquid handling device, methods of operating a liquid handling device, a method of performing a diagnostic test, a computer program and a system. In particular, the liquid handling device is capable of controllable bi-directional or multi-directional flow of reagents across one or more reaction zones allowing rapid, precise and controllable quenching of reactions and/or biological interactions.
Claims25
A liquid handling device comprising:
- a sample chamber for receiving a sample;
- a measurement chamber for performing one or more measurements on the sample wherein the measurement chamber comprises a reaction zone;
- a first liquid reagent chamber;
- a sample chamber conduit which fluidically connects the sample chamber to the measurement chamber;
- a sample chamber conduit valve for opening and closing the sample chamber conduit;
- a first liquid reagent chamber conduit which fluidically connects the first liquid reagent chamber to the measurement chamber in an alternate flow direction to the sample chamber conduit;
- and
- a first liquid reagent chamber conduit valve for opening and closing the first liquid reagent chamber conduit.
The liquid handling device of any previous claim, wherein the device further comprises:
- a second liquid reagent chamber;
- a second liquid reagent chamber conduit which fluidically connects the second liquid reagent chamber to the measurement chamber in an alternate flow direction to the sample chamber conduit;
- and
- a second liquid reagent chamber conduit valve for opening and closing the second liquid reagent chamber conduit.
The liquid handling device of any previous claim wherein the device comprises two or more measurement chambers, each of which is fluidically connected to the sample chamber and each of which is fluidically connected to the first liquid reagent chamber,
- wherein the device comprises a corresponding number of sample chamber conduit valves and/or first liquid reagent chamber valves for independent control of the flow of sample liquid and/or first liquid reagent into each measurement chamber.
The liquid handling device of any previous claim, wherein the flow of any one or more of the sample liquid, first liquid reagent and/or the second liquid reagent into each of the measurement chambers can be independently controlled to regulate the residence time of each liquid in each of the measurement chambers.
The liquid handling device of any previous claim, wherein the device further comprises:
- a mixing zone located between the sample chamber and the measurement chamber and wherein the mixing zone is fluidically connected to both the sample chamber and the measurement chamber.
A method of performing a diagnostic assay comprising sequentially moving liquid from a sample chamber to a measurement chamber and moving a first liquid reagent into the measurement chamber from an alternate flow direction, the method including:
- filling the sample chamber with sample liquid;
- moving sample liquid from the sample chamber to the measurement chamber;
- retaining the sample liquid in the measurement chamber for a predetermined period of time,
- moving a first liquid reagent from a first liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample chamber liquid and taking a measurement, optionally wherein the first liquid reagent is retained in the measurement chamber for a predetermined period of time.
A method of performing a diagnostic assay comprising sequentially moving liquid from a sample chamber to a measurement chamber and moving a first and second liquid reagent into the measurement chamber from an alternate flow direction, the method including:
- filling the sample chamber with sample liquid;
- moving sample liquid from the sample chamber to the measurement chamber;
- retaining the sample liquid in the measurement chamber for a predetermined period of time,
- moving a first liquid reagent from a first liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample liquid;
- moving a second liquid reagent from a second liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample liquid and performing a measurement, optionally wherein the first and second liquid reagents are each retained in the measurement chamber for a predetermined period of time.
A method of performing a diagnostic assay comprising sequentially moving liquid from a sample chamber to a measurement chamber and moving a first and second liquid reagent into the measurement chamber from an alternate flow direction, the method including:
- filling the sample chamber with sample liquid;
- moving sample liquid from the sample chamber to the measurement chamber;
- retaining the sample liquid in the measurement chamber for a predetermined period of time,
- moving a first liquid reagent from a first liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample liquid;
- moving a second liquid reagent from a second liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample liquid;
- moving a further volume of the first liquid reagent from the first liquid reagent chamber into the measurement chamber in an alternate flow direction to the sample liquid and performing a measurement, optionally wherein the first and second liquid reagents are each retained in the measurement chamber for a predetermined period of time.
Dependencies are inferred from the claim text.