Compositions and Methods for Analyte Detection
AssigneePresident and Fellows of Harvard College
InventorsDaniel Levner, Je-Hyuk Lee, George M. Church, Michael Super
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Abstract
The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.
Claims117
A method for detecting a plurality of analytes in a sample, comprising:
- a. contacting the sample with a composition comprising a plurality of detection reagents, wherein each subpopulation of the detection reagents targets at least one different analyte, and wherein each detection reagent comprises:
- at least one probe reagent targeting an analyte and at least one nucleic acid label comprising one or a plurality of pre-determined subsequences, wherein said at least one probe reagent and said at least one nucleic acid label are conjugated together;
- and wherein at least a portion of said one or the plurality of pre-determined subsequences form an identifier of said at least one probe reagent;
- and
- b. detecting in a temporally-sequential manner said one or the plurality of the pre-determined subsequences of said detection reagent, wherein said detection of the subsequences each generates a signal signature corresponding to said subsequence, and wherein a temporal order of the signal signatures corresponding to said one or the plurality of the subsequences of said detection reagent identifies a subpopulation of the detection reagents.
A detection reagent comprising at least one probe reagent and at least one nucleic acid label,
- wherein said at least one nucleic acid label comprises at least one pre-determined subsequence to be detected in a temporally-sequential manner;
- wherein said at least one pre-determined subsequence forms an identifier of said at least one probe reagent;
- and
- wherein said at least one probe reagent and said at least one nucleic acid label are conjugated together.
Dependencies are inferred from the claim text.