Deterministic lateral displacement array with a single column of bumping obstacles
AssigneeThe Trustees of Princeton University
InventorsWeibin LIANG, James C. STURM
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Abstract
Provided are microfluidic sorting devices comprising: a sample inlet, a single column comprising a plurality of bumping features configured for lateral displacement situated in a microfluidic channel, and a plurality of outlets, wherein the single column creates a main channel and a secondary channel in the microfluidic channel, wherein the sample inlet, the plurality of outlets, and the main channel and secondary channel are in fluid connection.
Claims84
A deterministic lateral displacement (DLD) device comprising a central channel and a single column of bumping obstacles configured for lateral displacement of particles in the central channel, wherein small particles flow out of the central channel to a small particle channel and large particles stay in the central channel and flow to a large particle outlet.
A microfluidic sorting device comprising:
- a central channel, a small particle channel;
- a single column of bumping obstacles configured for lateral displacement of particles in the central channel and a plurality of outlets, wherein the central channel, the sample inlet, the small particle channel and the plurality of outlets are fluidically connected.
The method of 52, wherein white blood cells comprise monocytes, T cells, B cells, regulatory T cells, central memory T cells, macrophages, dendritic cells, granulocytes, innate lymphoid cells, natural killer cells, or combinations thereof.
The method of 62, wherein the wash fluid further comprises:
- a) reagents that chemically react with the sample or other components of the wash fluid;
- or
- b) antibodies, carriers, or activators that interact with specific target cells or target particles.
The method of 64, wherein said method is used to concentrate cells sufficiently to allow for their administration to a patient without the need for centrifugation.
The method of 68, wherein the target cells or target particles make up at least 70% of the total particles in the final product.
A microfluidic device for separating particles based on their size, comprising:
- a) a central channel connected to a sample inlet at one end and to a large particle outlet located distally to, and fluidically connected with, the sample inlet;
- b) a buffer channel connected to a buffer inlet wherein the buffer channel is fluidically connected to the central channel by one or more laterally oriented buffer conduits;
- c) a small particle channel fluidically connected to the central channel by one or more laterally oriented sample fluid conduits and fluidically connected to a small particle outlet;
- d) a single column of bumping obstacles located between the central channel and small particle channel.
The method of either 79 or 80, wherein the particles smaller than the critical size of the microfluidic device are platelets or erythrocytes.
Dependencies are inferred from the claim text.