Methods of enhancing stem cell differentiation into beta cells
AssigneeVertex Pharmaceuticals Incorporated
InventorsFelicia PAGLIUCA, George HARB, Mads GURTLER, Austin THIEL, Jihad YASIN, Evrett THOMPSON
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Abstract
Disclosed herein are compositions and methods of enhancing stem cell differentiation into beta cells with use of one or more epigenetic modification compounds. The present disclosure also relates to compositions and methods of sorting and enriching the differentiated beta cells. The present disclosure also relates to compositions and methods of irradiating cell population for reducing proliferation.
Claims27
A method of treating a subject with Type 1 Diabetes, comprising administering to the subject a population of cells derived from stem cells in vitro, wherein:
- a) at least 35% of the cells in the population are NKX6.1-positive and ISL1-positive cells;
- b) at most 20% of the cells in the population are NKX6.1-negative and ISL1-negative cells;
- c) at least 60% of the cells in the population are CHGA-positive cells;
- and
- d) at least 30% of the cells in the population are C-peptide-positive cells.
Dependencies are inferred from the claim text.