Method for producing enhanced anti-inflammatory / anti-catabolic agents from autologous physiological fluid

A method of treating a mammal suffering from damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders with an autologous anti-inflammatory/anti-catabolic composition, the method comprising the following steps:

• admixing blood from the mammal with a quantity of sodium citrate to form an admixture;
• incubating the admixture at a temperature of from about 20° C. to about 40° C. for at least about 3.5 hours to about 12 hours;
• centrifuging the incubated admixture to separate the blood into a supernatant component and a cellular fraction;
• collecting the supernatant component to provide the autologous anti-inflammatory/anti-catabolic composition;
• and
• administering the autologous anti-inflammatory/anti-catabolic composition to the mammal.

A method of producing an autologous composition useful in the treatment of a mammal suffering from damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears, chronic degenerative joint conditions, and/or skin inflammatory disorders, the method comprising the following steps:

• preparing an anti-inflammatory/anti-catabolic component of the autologous composition comprising IL-1ra and TIMPs, said step of preparing the anti-inflammatory/anti-catabolic component comprising the following steps:
• admixing blood from the mammal with a quantity of sodium citrate to form an admixture;
• incubating the admixture at a temperature of from about 20° C. to about 40° C. for at least about 3.5 hours to about 12 hours;
• centrifuging the incubated admixture to separate the blood into a supernatant component and a cellular fraction;
• collecting the supernatant component of the anti-inflammatory/anti-catabolic component;
• preparing a regenerative component of the autologous composition comprising the following steps:
• mixing blood from the mammal with a quantity of an anticoagulant;
• centrifuging the blood to separate a platelet rich plasma component therefrom;
• collecting the platelet rich plasma component;
• and
• mixing the supernatant component of the anti-inflammatory/anti-catabolic component with the platelet rich plasma component to provide the autologous composition.

A method of treating a mammal suffering from damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders, the method comprising the following steps:

• preparing an anti-inflammatory/anti-catabolic component an autologous composition comprising IL-1ra and TIMPs, said step of preparing the anti-inflammatory/anti-catabolic component comprising the following steps:
• admixing blood from the mammal with a quantity of sodium citrate to form an admixture;
• incubating the admixture at a temperature of from about 20° C. to about 40° C. for at least about 3.5 hours to about 12 hours;
• centrifuging the incubated admixture to separate the blood into a supernatant component and a cellular fraction;
• and
• collecting the supernatant component of the anti-inflammatory/ anti-catabolic component;
• preparing a regenerative component of the autologous composition comprising the following steps:
• mixing blood from the mammal with a quantity of an anticoagulant;
• centrifuging the blood to separate a platelet rich plasma component therefrom;
• collecting the platelet rich plasma component;
• mixing the supernatant component with the platelet rich plasma component to provide the autologous composition;
• and
• administering the autologous composition to the mammal.