Microbial host cells for production of steviol glycosides
AssigneeManus Bio Inc.
InventorsRyan PHILIPPE, Ajikumar PARAYIL KUMARAN, Christine Nicole S. SANTOS
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Abstract
The present invention provides engineered cells and methods for making high purity steviol glycosides, including RebM. In some aspects, the present invention provides host cells, such as bacterial cells (including but not limited to E. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
Claims24
A method for making a target steviol glycoside composition, comprising:
- culturing an engineered microbial cell producing one or more target steviol glycosides, wherein the engineered microbial cell comprises recombinant expression of one or more transport proteins that transport the target steviol glycosides into the extracellular medium, and
- recovering the target steviol glycosides from the extracellular medium.
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An engineered host cell producing one or more target steviol glycosides, wherein the engineered bacterial cell comprises recombinant expression of one or more transport proteins that transport the target steviol glycosides into the extracellular medium.
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Dependencies are inferred from the claim text.