Self-assembled copolymeric 3d nanowire scaffold for cell growth and proliferation, and a method for producing thereof
AssigneeKing Saud University
InventorsSalman Alrokayan, Fouzi Mouffouk, Haseeb Ahmad Khan, Tajamul Hussain, Salman Alamery, Khalid M. Abu-Salah
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Abstract
The present disclosure pertains to a 3D scaffold for cell growth and proliferation. In particular, the present disclosure provides a method of producing an artificial 3D scaffold to support stem cell growth and later their differentiation, by converting biodegradable amphiphilic copolymers (star polymer) into nanowire scaffolds, through a molecular self-assembly process. The invention also relates to the use of said scaffold for cell culture and/or transplantation.
Claims18
A method for fabricating biodegradable copolymeric 3D nanowire scaffold to support cell growth and later their differentiation, said method comprises the steps of:
- a. synthesizing polycaprolactone (PCL) macromonomer units;
- b. synthesizing poly(ethylene glycol) (PEG) macromonomer units;
- c. polymerizing the PCL macromonomer units and the PEG macromonomer units with a compound 1 having the structure:
- by Atom transfer radical polymerization (ATRP) polymerization to obtain an amphiphilic copolymer, poly(caprolactone)-b-poly(ethylene oxide) star-polymer;
- d. providing PEG-fibronectin;
- e. bioconjugation of poly(caprolactone)-b-poly(ethylene oxide) star-polymer with fibronectin by mixing poly(caprolactone)-b-poly(ethylene oxide) star-polymer with PEG-fibronectin in DMF, followed by stirring;
- f. adding deionized water in frequent intervals, followed by dialysis against deionized water effecting the formation of biodegradable copolymeric 3D nanowire scaffold (poly(star)-Fibronectin nanowires) by a molecular self-assembly process,
- wherein, the molecular self-assembly process is effected by the solvent removal procedure by dialysis, whereby slow removal of the organic phase triggers micellization that later leads to the formation of the nanowires;
- the concentration of the amphiphilic copolymer;
- and the molecular structure of the amphiphilic copolymer.
Dependencies are inferred from the claim text.