Preparation of neosaxitoxin
AssigneeThe Cawthron Institute Trust Board
InventorsANDREW IAN SELWOOD, ROELOF VAN GINKEL, ALAN CRAIG WAUGH
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Abstract
A semisynthetic method of preparing neosaxitoxin from cultures of the dinoflagellate Gymnodinium catenatum is described. The scalable method includes the reductive desulfonation of an unresolved mixture of toxin C3 and toxin C4 and mild acid hydrolysis of the resulting gonyautoxin 6 (GTX6) to provide the neosaxitoxin.
Claims12
A method of preparing neosaxitoxin comprising the mild acid hydrolysis of a quantity of GTX6 obtained by the reductive desulfonation of a quantity of C3,4 purified from an extract of a culture of an isolate of Gymnodinium catenatum where the reductive desulfonation is by contacting in a solution in a reaction solvent the quantity of C3,4 and a quantity of dithiol at a temperature and for a time sufficient to provide a reaction product in which greater than 97.5% (w/w) of the quantity of C3,4 has been converted to GTX6 to provide the quantity of GTX6.
Dependencies are inferred from the claim text.