Luciferase linked immunosorbent assay
AssigneeInstitut Pasteur
InventorsThierry Rose, Sophie GOYARD, Laurent Lionel REBER, Yves JANIN
Life of this patent
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Abstract
The present invention relates to a fusion protein comprising: —a N-terminal domain which comprises an antibody which is a variable domain of a camelid heavy-chain antibody (VHH) or a single chain variable fragment (scFV) and which is directed against an immunoglobulin and —a C-terminal domain which comprises a polypeptide with a luciferase activity: —having the amino acid sequence SEQ ID NO: 1 or —having at least 80% amino acid sequence identity to the amino acid sequence SEQ ID NO: 1.
Claims17
A fusion protein comprising:
- a N-terminal domain which comprises an antibody which is a variable domain of a camelid heavy-chain antibody (VHH) or a single chain variable fragment (scFV) and which is directed against an immunoglobulin and
- a C-terminal domain which comprises a polypeptide with a luciferase activity:
- having the amino acid sequence SEQ ID NO:
- 1 or
- having at least 80% amino acid sequence identity to the amino acid sequence SEQ ID NO:
- 1.
A luciferase having at least 80% amino acid sequence identity to the amino acid sequence SEQ ID NO:
- 1 and comprising at least one amino acid substitution selected from the group consisting of:
- substitution of the tyrosine (Y) at a position corresponding to the position 18 of SEQ ID NO:
- 1 with an arginine (R),
- substitution of the leucine (L) at a position corresponding to the position 48 of SEQ ID NO:
- 1 with a lysine (K),
- substitution of the isoleucine (I) at a position corresponding to the position 56 of SEQ ID NO:
- 1 with an alanine (A),
- substitution of the tyrosine (Y) at a position corresponding to the position 116 of SEQ ID NO:
- 1 with a phenylalanine (F),
- substitution of the tryptophan (W) at a position corresponding to the position 134 of SEQ ID NO:
- 1 with an amino acid selected from the group consisting of a threonine (T) and glutamate (E),
- substitution of the tryptophan (W) at a position corresponding to the position 163 of SEQ ID NO:
- 1 with an amino acid selected from the group consisting of a threonine (T) and glutamate (E),
- and
- substitution of the cysteine (C) at a position corresponding to the position 166 of SEQ ID NO:
- 1 with a serine (S).
A luciferase having an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, and SEQ ID NO 17.
Dependencies are inferred from the claim text.